Identificación molecular y perfil de sensibilidad antifúngica de Candida albicans y Candida dubliniensis aisladas de pacientes ambulatorios y hospitalizados

Carolina Duré; Margarita Samudio; Norma Fariña; Sonia Abente; Alicia Pereira; Rosa Guillén; Gustavo Aguilar; Belén Gayoso; Heriberto Nuñez; Desiré Almirón; Aurelia Taboada

doi:10.52379/mcs.v8i3.431

Identificación molecular y perfil de sensibilidad antifúngica de Candida albicans y Candida dubliniensis aisladas de pacientes ambulatorios y hospitalizados

Authors

Carolina Duré Universidad Nacional de Asunción, Instituto de Investigaciones en Ciencias de la Salud, San Lorenzo, Paraguay https://orcid.org/0000-0001-5886-2357
Margarita Samudio Universidad Nacional de Asunción, Instituto de Investigaciones en Ciencias de la Salud, San Lorenzo, Paraguay https://orcid.org/0000-0003-2813-218X
Norma Fariña Laboratorio San Roque, Asunción, Paraguay https://orcid.org/0000-0002-8816-1786
Sonia Abente Universidad Nacional de Asunción, Instituto de Investigaciones en Ciencias de la Salud, San Lorenzo, Paraguay https://orcid.org/0000-0002-0263-4205
Alicia Pereira Laboratorio San Roque, Asunción, Paraguay https://orcid.org/0000-0003-4021-5762
Rosa Guillén Universidad Nacional de Asunción, Instituto de Investigaciones en Ciencias de la Salud, San Lorenzo, Paraguay https://orcid.org/0000-0002-4778-4960
Gustavo Aguilar Instituto de Previsión Social, Asunción, Paraguay https://orcid.org/0000-0002-1535-4149
Belén Gayoso Universidad Nacional de Asunción, Instituto de Investigaciones en Ciencias de la Salud, San Lorenzo, Paraguay https://orcid.org/0000-0001-8315-8273
Heriberto Nuñez Universidad Nacional de Asunción, Facultad de Odontología, Asunción, Paraguay
Desiré Almirón Instituto de Medicina Tropical, Programa Nacional de Control de VIH/Sida e ITS (PRONASIDA), Asunción, Paraguay https://orcid.org/0000-0001-7173-3461
Aurelia Taboada Instituto de Medicina Tropical, Programa Nacional de Control de VIH/Sida e ITS (PRONASIDA), Asunción, Paraguay

DOI:

https://doi.org/10.52379/mcs.v8i3.431

Keywords:

Candida albicans, Candida dubliniensis, Polymerase Chain Reaction, Microbial Susceptibility Tests, Antifungal Agents

Abstract

Introduction: C. albicans together with C. dubliniensis and C. Africana, form the Candida albicans Complex. These species are closely related to each other, making differentiation by conventional methods difficult. Objectives: To differentiate Candida albicans and Candida dubliniensis by molecular method in isolates from outpatients and hospitalized patients from Central, Paraguay and to know their susceptibility profile. Methods: Yeasts isolated from respiratory materials, oral cavity, purulent secretions, blood, urine and others were included. An end-point duplex PCR was performed on the isolates that developed greenish coloration in chromogenic medium (CONDA®, Spain). DNA was extracted using the Wizard® Genomic DNA Kit (Promega, USA) with some modifications. Antifungal susceptibility was determined by VITEK® 2 to a subgroup of isolates. Results: Of 1065 isolates, 838 (78,7 %) were C. albicans and 3 (0,3 %) C. dubliniensis, the latter coming from the oral cavity; the remaining 224 were negative for both species. 94,4 % of 503 C. albicans isolates were susceptible to fluconazole, 97,6 % to voriconazole, 99,4 % to amphotericin B, 100 % to caspofungin and micafungin. The three isolates of C. dubliniensis presented MICs of ? 0,5 µg/mL against fluconazole, ? 0,12 µg/mL against voriconazole and ? 0,25 µg/mL against amphotericin B. Conclusions: The frequency of C. dubliniensis is low compared to other studies that report values ??of 1,5 to 32 %. These isolates did not exhibit signs of resistance. C. albicans exhibited good susceptibility to antifungals tested.

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